The 1-base overhangs produced by EcoNI may be hard to ligate. Genome Compiler Vector NTI Serial Cloner. Benchling is a life science data management and collaboration platform. To install to an alternative location run the SnapGene installer use the /S. If you want to install and use SnapGene on another PC (e.g. Alternatives to SnapGene Viewer Benchling. Snapgene Student License The age classes confirm the difference in the. Prolonged incubation with NdeI may lead to removal of additional nucleotides.Įfficient cleavage requires at least two copies of the SgrAI recognition sequence.Ĭ C T N N N N N A G G G G A N N N N N T C C The alternative version Adobe Acrobat Pro DC is used for this. Sticky ends from different BlpI sites may not be compatible.Īfter cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. Sticky ends from different Esp3I sites may not be compatible. Ventral root avulsion also induced a decrease in levels of SNAP-25 RNA transcripts, suggesting that the axonal injury in itself was responsible for the down-regulation of Snap gene expression. A significant decrease was demonstrated 2 days after axotomy, which reached a maximum after 7 days (62% for SNAP-25a and 67% for SNAP-25b), while levels had slightly recovered by 14 and 28 days. After unilateral sciatic nerve transection (axotomy), SNAP-25a and SNAP-25b expression decreased in axotomized motoneurons compared with corresponding motoneurons on the unlesioned side. Cloning of the rat Snap gene intron spacing the alternative exon 5a and 5b sequences and generation of an intron-specific oligonucleotide probe used for in situ hybridization did not point to the presence of unspliced variants of SNAP-25b mRNA. In all animals, SNAP-25a RNA transcripts were demonstrated in the nucleus of motoneurons, whereas SNAP-25b mRNA was present mainly in the cytoplasm. The effect of unilateral nerve injury on SNAP-25 mRNA levels was studied in motoneurons of the rat lumbar spinal cord. In situ hybridization was used to examine whether SNAP-25 isoform mRNA expression may be altered by experimental manipulations. SNAP-25 exists in two isoforms, which arise from alternative splicing of exon 5. Synaptosomal-associated protein of 25 kDa (SNAP-25) is involved in the molecular regulation of neurotransmitter release.
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